LiDirect™ Lightning Genotyping kit is a combination of LiDirect™ Genotyping DNA Extraction Buffer and LiTaq™ Plus PCR master mix. It is designed for fast extraction of tissue DNA and subsequent PCR analysis. LiDirect™ Genotyping kit contains all the reagents needed to rapidly (15 min) extract genomic DNA from mouse tails, yeast, plant cells and other tissues, cells, hair shafts, and saliva. It also include all reagents for subsequent genotyping PCR. The whole genotyping process can be completed within 1 hour.
Key Features
• The procedure is fast and simple, amenable to automation. The entire process takes less than 1 hour.
• Does not use hazardous chemicals such as phenol, chloroform, proteinase K or guanidine isothiocyanate.
• There is no need for mechanical disruption, organic extraction, column purification, or precipitation of the DNA.
Components
1. LiDirect™ Extraction Buffer: 40 ml
2. LiDirect™ Stop Solution: 40 ml
3. 2× LiTaq™ Plus PCR Master Mix: 5×1 ml
Storage
PCR Master Mix: -20°C; Extraction Buffer & Stop Solution: 4-25°C.
Case Study
Taking advantage of our new proprietary formula, the LiDirect™ DNA extraction buffer enables fast DNA release from the toughest cells, and performs much better than traditional alkaline lysis protocols. Combined with our industry leading LiTaq™ Plus DNA polymerase, our LiDirect™ Lightning Genotyping Kit helps scientists to genotype all types of tissues and cells effortlessly.
| Step 1: sample preparation • 1~2 mm piece of mouse tail • 5~10 mg of tissue • ~1×104 cells Step 2: add 75 µl of LiDirect™ Extraction Buffer • Incubate at 98°C for 15 min Step 3: add 125 µl of LiDirect™ Stop Solution Step 4: set up 25 µl of LiTaq™ Plus PCR reaction mix |
PCR cycling for amplification: 1. 95°C: 15 sec 2. 35 × • Denaturation: 5 sec at 98°C • Annealing: 10 sec at 55°C • Extension: 30 sec/kb at 72°C 3. Final extension: 5 min at 72°C |
Comparison of genotyping PCR results with Taq polymerase from different vendors
Template is mouse genomic DNA extracted with the LiDirect™ DNA extraction buffer (M0014).
Lanes 1-3 are our Taq products: LiTaq™ Eco PCR Master Mix (M0034), LiTaq™ PCR Master Mix (M0024), LiTaq™ Plus PCR Master Mix (M0025).
Lanes 4-10 are competitor brands.
From these results, it can be concluded that our LiDirect™ DNA extraction buffer is compatible with most Taq polymerases on the market, but our LiTaq™ plus product line is more robust than most other products on market.
